Recombinant
RabMAb

Anti-TSFM antibody [EPR12034] (ab173528)

Overview

  • Product name
    Anti-TSFM antibody [EPR12034]
    See all TSFM primary antibodies
  • Description
    Rabbit monoclonal [EPR12034] to TSFM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TSFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P43897

  • Positive control
    • HeLa, Jurkat, 293T and HepG2 cell lysates. HeLa cells. Immunoprecipitation pellet from 293T cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
    • Tissue specificity
      Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.
    • Involvement in disease
      Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3) [MIM:610505]. Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes.
    • Sequence similarities
      Belongs to the EF-Ts family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • COXPD 3 antibody
      • COXPD3 antibody
      • EF TS antibody
      • EF Tsmt antibody
      • EF-Ts antibody
      • EF-TsMt antibody
      • EFTS antibody
      • EFTS_HUMAN antibody
      • EFTSMT antibody
      • Elongation factor Ts antibody
      • Elongation factor Ts mitochondrial antibody
      • HGNC12367 antibody
      • mitochondrial antibody
      • Mitochondrial elongation factor Ts antibody
      • Ts translation elongation factor mitochondrial antibody
      • TSFM antibody
      • TSMT antibody
      see all

    Images

    • All lanes : Anti-TSFM antibody [EPR12034] (ab173528) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunofluorescence analysis of HeLa cells labeling TSFM with ab173528 at a 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from 293T cell lysate:  ab173528 at a 1/10 dilution was used to immunoprecipitated TSFM.

    References

    ab173528 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Hippocampus)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    1 µg
    Specification
    Hippocampus
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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