Anti-TSG101 antibody (ab225877)

Rabbit polyclonal TSG101 antibody. Validated in WB and tested in Human. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to TSG101
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human TSG101 aa 225-275. The exact sequence is proprietary. (NP_006283.1).
    Database link: Q99816

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab225877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.
    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Information by UniProt
    • Database links

    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • All lanes : Anti-TSG101 antibody (ab225877) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 44 kDa


      Exposure time: 3 minutes

    References

    ab225877 has not yet been referenced specifically in any publications.

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