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    tsg101-antibody-ab225877.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Other
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Anti-TSG101 antibody (ab225877)

  • Datasheet
  • SDS
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Western blot - Anti-TSG101 antibody (ab225877)

    Key features and details

    • Rabbit polyclonal to TSG101
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-TSG101 antibody
      See all TSG101 primary antibodies
    • Description

      Rabbit polyclonal to TSG101
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Orangutan, Xenopus tropicalis
    • Immunogen

      Synthetic peptide within Human TSG101 aa 225-275. The exact sequence is proprietary. (NP_006283.1).
      Database link: Q99816

    • Positive control

      • WB: HeLa, HEK-293T and Jurkat whole cell lysate (ab7899).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.1% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab225877 was affinity purified using an epitope specific to TSG101 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Other
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • Other
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Differentiation
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Cancer
      • Cell cycle
      • Cell cycle inhibitors
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab225877 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
    Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 44 kDa.

    Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.
    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Target information above from: UniProt accession Q99816 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 423082 Chicken
      • Entrez Gene: 451065 Chimpanzee
      • Entrez Gene: 507659 Cow
      • Entrez Gene: 485406 Dog
      • Entrez Gene: 100057099 Horse
      • Entrez Gene: 7251 Human
      • Entrez Gene: 100172234 Orangutan
      • Entrez Gene: 100524238 Pig
      • Entrez Gene: 100343429 Rabbit
      • Entrez Gene: 446571 Xenopus laevis
      • Omim: 601387 Human
      • SwissProt: Q99816 Human
      • Unigene: 523512 Human
      see all
    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Western blot - Anti-TSG101 antibody (ab225877)
      Western blot - Anti-TSG101 antibody (ab225877)
      All lanes : Anti-TSG101 antibody (ab225877) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 44 kDa


      Exposure time: 3 minutes

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab225877? Please let us know so that we can cite the reference in this datasheet.

    ab225877 has been referenced in 2 publications.

    • Tong L  et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics 11:8570-8586 (2021). PubMed: 34373759
    • O'Dowd K  et al. Distinct miRNA Profile of Cellular and Extracellular Vesicles Released from Chicken Tracheal Cells Following Avian Influenza Virus Infection. Vaccines (Basel) 8:N/A (2020). PubMed: 32764349

    Customer reviews and Q&As

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