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Anti-TSG101 antibody (ab245448)

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Western blot - Anti-TSG101 antibody (ab245448)

    Key features and details

    • Rabbit polyclonal to TSG101
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-TSG101 antibody
      See all TSG101 primary antibodies
    • Description

      Rabbit polyclonal to TSG101
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human TSG101 aa 300-350. The exact sequence is proprietary. (NP_006283.1).
      Database link: Q99816

    • Positive control

      • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab245448 was affinity purified using an epitope specific to TSG101 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Other
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • Other
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Differentiation
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Cancer
      • Cell cycle
      • Cell cycle inhibitors
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • NIH/3T3 whole cell lysate (ab7179)
      • Jurkat whole cell lysate (ab7899)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab245448 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Application Abreviews Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
    Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 44 kDa.

    Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.
    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Target information above from: UniProt accession Q99816 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7251 Human
      • Entrez Gene: 22088 Mouse
      • Entrez Gene: 292925 Rat
      • Omim: 601387 Human
      • SwissProt: Q99816 Human
      • SwissProt: Q61187 Mouse
      • SwissProt: Q6IRE4 Rat
      • Unigene: 523512 Human
      • Unigene: 241334 Mouse
      • Unigene: 7410 Rat
      see all
    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Western blot - Anti-TSG101 antibody (ab245448)
      Western blot - Anti-TSG101 antibody (ab245448)
      All lanes : Anti-TSG101 antibody (ab245448) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 44 kDa


      Exposure time: 3 minutes

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab245448? Please let us know so that we can cite the reference in this datasheet.

    ab245448 has not yet been referenced specifically in any publications.

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