Name: Anti-TSG101 antibody [EPR7130(B)]
Brand: RabMAb
SKU: ab125011
Rating: 4 (9 reviews)

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Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7130(B)] to TSG101
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free PE

Product name

Anti-TSG101 antibody [EPR7130(B)]
See all TSG101 primary antibodies
Description

Rabbit monoclonal [EPR7130(B)] to TSG101
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: K562, Jurkat, and SH-SY5Y cell lysates. Human brain lysate; mouse and rat heart tissue lysates. IHC-P: Human kidney tissue. ICC: HEK-293 cells. Flow Cyt (intra): HeLa and K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 4.00 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7130(B)
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(6)	1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt (Intra)		1/40. For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF	(3)	1/100. For unpurified use at 1/10.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt (Intra) 1/40. For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100. For unpurified use at 1/10.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30.

Application notes

Is unsuitable for IP.

Function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Tissue specificity

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain.
Domain

The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
Post-translational
modifications

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Cellular localization

Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
Target information above from: UniProt accession Q99816 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7251 Human
- Entrez Gene: 22088 Mouse
- Entrez Gene: 292925 Rat
- Omim: 601387 Human
- SwissProt: Q99816 Human
- SwissProt: Q61187 Mouse
- SwissProt: Q6IRE4 Rat
- Unigene: 523512 Human
- Unigene: 241334 Mouse
- Unigene: 7410 Rat
see all
Alternative names
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
- TSG 10 antibody
- TSG 101 antibody
- TSG10 antibody
- Tsg101 antibody
- Tumor susceptibility 101 antibody
- Tumor susceptibility gene 10 antibody
- Tumor susceptibility gene 101 antibody
- Tumor susceptibility gene 101 protein antibody
- Tumor susceptibility protein antibody
- Tumor susceptibility protein isoform 3 antibody
- VPS 23 antibody
- VPS23 antibody
see all

Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Human brain lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Intracellular flow cytometric analysis of permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.
OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Different batches of ab125011 were tested on Mouse heart lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 45 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Intracellular Flow Cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) (purified)

All lanes : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Intracellular Flow Cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab125011? Please let us know so that we can cite the reference in this datasheet.

ab125011 has been referenced in 143 publications.

Lin F et al. Bladder cancer cell-secreted exosomal miR-21 activates the PI3K/AKT pathway in macrophages to promote cancer progression. Int J Oncol 56:151-164 (2020). PubMed: 31814034
Liang Y et al. Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. J Extracell Vesicles 9:1697583 (2020). PubMed: 31853339
Han M et al. Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer. Cell Death Dis 11:43 (2020). PubMed: 31969559
Brennan K et al. A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum. Sci Rep 10:1039 (2020). PubMed: 31974468
Zhao Y et al. Liver governs adipose remodelling via extracellular vesicles in response to lipid overload. Nat Commun 11:719 (2020). PubMed: 32024826

View all Publications for this product

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1-9 of 9 Abreviews or Q&A

Application

Western blot

Sample

Bos taurus Cell lysate - other (exosomes)

Gel Running Conditions

Reduced Denaturing (4-12% Nupage Gel)

Loading amount

10 µg

Specification

exosomes

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

Paula Mitchell

Verified customer

Submitted Jun 03 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (MC3T3 cells)

Permeabilization

Yes - NP40

Specification

MC3T3 cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 06 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (U2OS)

Permeabilization

Yes - NP40

Specification

U2OS

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Nov 06 2020

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

25 µg

Specification

Mouse vascular smooth muscle cells

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 14 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (U2OS)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

U2OS

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 14 2020

Application

Western blot

Sample

Bos taurus Purified protein (Extracellular vesicle (EV) enriched whey fraction)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris gel)

Loading amount

20 µg

Specification

Extracellular vesicle (EV) enriched whey fraction

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jul 27 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (U266)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

U266

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 02 2018

Application

Western blot

Sample

Human Cell lysate - other (Exosomes from HK-2 cells)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

50 µg

Specification

Exosomes from HK-2 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Eric Barros

Verified customer

Submitted Oct 24 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Breast Cancer Cell Line MDA-MB231)

Permeabilization

Yes - 0.1% Triton

Specification

Breast Cancer Cell Line MDA-MB231

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C

Fixative

Paraformaldehyde

Mr. Gabriel Ortega

Verified customer

Submitted Mar 06 2017

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For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (143)

Customer reviews and Q&As

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Western blot abreview for Anti-TSG101 antibody [EPR7130(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TSG101 antibody [EPR7130(B)]

Dissociation constant (K_D)

Post-translational
modifications