RecombinantRabMAb

Recombinant Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)

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Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR7130(B)] to TSG101 - Low endotoxin, Azide free
    • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)
    • Reacts with: Mouse, Rat, Human

    Overview

    • Product name

      Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free
      See all TSG101 primary antibodies

    • Description

      Rabbit monoclonal [EPR7130(B)] to TSG101 - Low endotoxin, Azide free

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • WB: mouse heart tissue lysates.

    • General notes

      ab216447 is the carrier-free version of ab125011.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

      We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab216447 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.

     
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    See IHC antigen retrieval protocols.
    Flow Cyt (Intra)
    Use at an assay dependent concentration.

    ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
    Notes
    WB
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.

     
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    See IHC antigen retrieval protocols.
    Flow Cyt (Intra)
    Use at an assay dependent concentration.

    ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.

    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)
      Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)

    References (0)

    Publishing research using ab216447? Please let us know so that we can cite the reference in this datasheet.

    ab216447 has not yet been referenced specifically in any publications.

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