Recombinant Anti-TSG101 antibody [EPR7131(B)] (ab133586)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Anti-TSG101 antibody [EPR7131(B)]
See all TSG101 primary antibodies -
Description
Rabbit monoclonal [EPR7131(B)] to TSG101 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TSG101 (C terminal). The exact sequence is proprietary.
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Positive control
- PC12, NIH 3T3, K562, 293T and Jurkat whole cell lysate (ab7899)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.81 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7131(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
- Jurkat nuclear extract lysate (ab14844)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- PC12 cytoplasmic extract lysate (ab14883)
- PC12 nuclear extract lysate (ab14884)
- Jurkat membrane extract lysate (ab30130)
- 293T whole cell lysate (ab95494)
Applications
Our Abpromise guarantee covers the use of ab133586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 44 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. -
Tissue specificity
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain. -
Domain
The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently. -
Post-translational
modificationsMonoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. -
Cellular localization
Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 7251 Human
- Entrez Gene: 22088 Mouse
- Entrez Gene: 292925 Rat
- Omim: 601387 Human
- SwissProt: Q99816 Human
- SwissProt: Q61187 Mouse
- SwissProt: Q6IRE4 Rat
- Unigene: 523512 Human
see all -
Alternative names
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
see all
Images
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![Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)](https://www.abcam.com/ps/products/133/ab133586/Images/ab133586-268180-anti-tsg101-antibody-epr7131b-western-blot.jpg)
Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/2000 dilution (purified)
Lane 1 : K562 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : PC-12 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)](https://www.abcam.com/ps/products/133/ab133586/Images/ab133586-268213-anti-tsg101-antibody-epr7131b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).
Lane 1 (input): K562 whole cell lysate 10μg
Lane 2 (+): ab133586 + K562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)](https://www.abcam.com/ps/products/133/ab133586/Images/ab133586-147396-anti-tsg101-antibody-epr7131b-western-blot.jpg)
Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/1000 dilution (unpurified)
Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 44 kDa
Note: Dilute primary antibody in 1% BSA. -
![Other - Anti-TSG101 antibody [EPR7131(B)] (ab133586)](https://www.abcam.com/ps/products/133/ab133586/Images/ab133586-147395-anti-tsg101-antibody-epr7131b.jpg)
Other - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
Datasheets and documents
References
This product has been referenced in:
- Yu J et al. Detection of Exosomal PD-L1 RNA in Saliva of Patients With Periodontitis. Front Genet 10:202 (2019). Read more (PubMed: 30923536) »
- Guix FX et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29495441) »
