Recombinant
RabMAb

Recombinant Anti-TSG101 antibody [EPR7131(B)] (ab133586)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7131(B)] to TSG101
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSG101 antibody [EPR7131(B)]
    See all TSG101 primary antibodies

  • Description

    Rabbit monoclonal [EPR7131(B)] to TSG101

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TSG101 (C terminal). The exact sequence is proprietary.
    Database link: 133586

  • Positive control

    • WB: PC12, NIH 3T3, K562, 293T and Jurkat whole cell lysate (ab7899). IP: K562 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab133586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.

    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/2000 dilution (purified)

      Lane 1 : K562 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : PC-12 whole cell lysate
      Lane 4 : NIH/3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)

      ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).

      Lane 1 (input): K562 whole cell lysate 10μg

      Lane 2 (+): ab133586 + K562 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/1000 dilution (unpurified)

      Lane 1 : PC12 cell lysate
      Lane 2 : NIH 3T3 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 44 kDa



      Note: Dilute primary antibody in 1% BSA.
    • Other - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      Other - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References (12)

    Publishing research using ab133586? Please let us know so that we can cite the reference in this datasheet.

    ab133586 has been referenced in 12 publications.

    • Yu J  et al. Detection of Exosomal PD-L1 RNA in Saliva of Patients With Periodontitis. Front Genet 10:202 (2019). PubMed: 30923536
    • Feng Y  et al. Exosome-derived miRNAs as predictive biomarkers for diffuse large B-cell lymphoma chemotherapy resistance. Epigenomics 11:35-51 (2019). PubMed: 30211623
    • Wu B  et al. Exosomes isolated from CAPS1-overexpressing colorectal cancer cells promote cell migration. Oncol Rep 42:2528-2536 (2019). PubMed: 31638236
    • Zeng X & Carlin CR Adenovirus early region 3 RIDa protein limits NF?B signaling through stress-activated EGF receptors. PLoS Pathog 15:e1008017 (2019). PubMed: 31425554
    • Guix FX  et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci 19:N/A (2018). PubMed: 29495441
    View all Publications for this product

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