Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7131(B)] to TSG101
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-TSG101 antibody [EPR7131(B)]
See all TSG101 primary antibodies
DescriptionRabbit monoclonal [EPR7131(B)] to TSG101
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TSG101 (C terminal). The exact sequence is proprietary.
Database link: 133586
- WB: PC12, NIH 3T3, K562, 293T and Jurkat whole cell lysate (ab7899). IP: K562 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 7.81 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||1/10 - 1/100.|
FunctionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain.
DomainThe UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
modificationsMonoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Cellular localizationCytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
- Information by UniProt
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/2000 dilution (purified)
Lane 1 : K562 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : PC-12 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Blocking/Diluting buffer 5% NFDM/TBST
ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).
Lane 1 (input): K562 whole cell lysate 10μg
Lane 2 (+): ab133586 + K562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/1000 dilution (unpurified)
Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 44 kDa
Note: Dilute primary antibody in 1% BSA.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133586 has been referenced in 12 publications.
- Yu J et al. Detection of Exosomal PD-L1 RNA in Saliva of Patients With Periodontitis. Front Genet 10:202 (2019). PubMed: 30923536
- Feng Y et al. Exosome-derived miRNAs as predictive biomarkers for diffuse large B-cell lymphoma chemotherapy resistance. Epigenomics 11:35-51 (2019). PubMed: 30211623
- Wu B et al. Exosomes isolated from CAPS1-overexpressing colorectal cancer cells promote cell migration. Oncol Rep 42:2528-2536 (2019). PubMed: 31638236
- Zeng X & Carlin CR Adenovirus early region 3 RIDa protein limits NF?B signaling through stress-activated EGF receptors. PLoS Pathog 15:e1008017 (2019). PubMed: 31425554
- Guix FX et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci 19:N/A (2018). PubMed: 29495441