Recombinant
RabMAb

Recombinant Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free (ab240070)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7131(B)] to TSG101 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free
    See all TSG101 primary antibodies

  • Description

    Rabbit monoclonal [EPR7131(B)] to TSG101 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TSG101 aa 350-450 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab240070 is the carrier-free version of ab133586. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240070 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab240070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.

    • Domain

      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

    • Post-translational
      modifications

      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

    • Cellular localization

      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free (ab240070)
      Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free (ab240070)

      ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).

      Lane 1 (input): K562 whole cell lysate 10μg

      Lane 2 (+): ab133586 + K562 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133586).

    • Other - Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free (ab240070)
      Other - Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free (ab240070)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133586).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240070? Please let us know so that we can cite the reference in this datasheet.

    ab240070 has not yet been referenced specifically in any publications.

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