Key features and details
- Rabbit polyclonal to TSGA14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TSGA14 antibody
DescriptionRabbit polyclonal to TSGA14
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
A region between residue 150 and 200 of human TSGA14 (NP_061188.1).
- HeLa and 293T whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to TSGA14 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired during ciliogenesis for tubulin glutamylation in cilium. Probably acts by participating to the transport of TTLL6, a tubulin polyglutamylase, between the basal body and the cilium.
Tissue specificityIsoform 1 and isoform 4 are expressed in testis and fetal tissues.
Involvement in diseaseDefects in CEP41 are the cause of Joubert syndrome type 15 (JBTS15) [MIM:614464]. JBTS15 is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy, renal disease, liver fibrosis and polydactyly.
Note=Genetic variations in CEP41 may be associated with susceptibility to autism.
Sequence similaritiesBelongs to the CEP41 family.
Contains 1 rhodanese domain.
DomainAlthough it contains a rhodanese domain, does not display phosphatase activity, suggesting that the protein is enzymatically inactive (PubMed:22246503).
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Localizes mainly to the cilium basal body and in primary cilia.
- Information by UniProt
- centrosomal protein 41 kDa antibody
- Centrosomal protein of 41 kDa antibody
- CEP 41 antibody
All lanes : Anti-TSGA14 antibody (ab86180) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 100 kDa, 200 kDa, 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Human TSGA14 by Immunoprecipitation, using ab86180 at 3µg/mg lysate. Image shows immunoprecipitated TSGA14 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab86180 has not yet been referenced specifically in any publications.