Overview

  • Product name
    Anti-TSH antibody [11E4]
    See all TSH primary antibodies
  • Description
    Mouse monoclonal [11E4] to TSH
  • Host species
    Mouse
  • Specificity
    This antibody specifically recognizes ß-subunit of human TSH.
  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) from human pituitary gland.

  • General notes
    This antibody (capture) is ELISA paired with ab6070 (conjugate).

Properties

Applications

Our Abpromise guarantee covers the use of ab6069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notes
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [7G12] to TSH (ab6070).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • CG ALPHA antibody
      • CGA antibody
      • Chorionic gonadotropin alpha polypeptide antibody
      • FSHA antibody
      • Glycoprotein hormones alpha polypeptide antibody
      • GPHA1 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyrotropin beta subunit antibody
      • TSH beta antibody
      • TSHA antibody
      • TSHB antibody
      see all

    References

    ab6069 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for your enquiry.

    The TSH protein ab110682 is purified human protein. So in theory it should be detected by any antibody which will cross react withhuman TSH. Although please make sure the antibody is also tested in the application of interest.

    I hope this is helpful. Please contact us again if you have any further questions.

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    Answer

    It is definitely my pleasure to help you with these issues. I look forward to hearing how the new product works in your experiments.

    We do not have the specific antigen used to create these products available on our catalog at this time. However we do offer a full length purified nativeTSH protein which is in its active form. That product number is AB110682 and can be found at the following link:

    https://www.abcam.com/TSH-protein-Active-ab110682.html

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Answer

    Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA

    Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

    In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

    The suggested secondary antibodies are:
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

    Testing Discount Codes:

    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in sELISA, mouse, rat
    DISCOUNT CODE: xxx-test in sELISA, mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat

    DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
    DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please let me know if there is anything else I can help you with.

    Read More

    Answer

    Thank you for your call this morning and for your patience.

    There is an error on the pdf form of our sandwich ELISA protocol, and the carbonate buffer should be around 9.6. I've looked through several protocols, and most recommend either a pH 9.6 carbonate buffer or a 7.4 PBS solution for coating. I think that the buffer you are currently using is fine, and I apologize for the mistake on the protocol.

    It's possible that a higher concentration of antibody or antigen will help increase the signal. Could you send me some more details about the protocol and results that you've seen, and I will see if there are any suggestions or explanations?

    I look forward to hearing from you. Please let me know if there is anything else that I can do for you and I'll be happy to help.

    Read More

    Answer

    Thank you for contacting us.

    I have have the chance to look over each of the TSH ELISA kits. Unfortunately it appears that none of the currently offered kits uses ab6069 or ab24921. It may be that the particular product that was recommended to you if no longer offered at Abcam.

    Each of our kits is covered by our Abpromise and is 100% guaranteed to work should they be used in a manner consistent with what we have published in the datasheet. However,should you to perform a sandwichELISA using ab6069 I have found that the the matched pair for ab6069 is actually ab6070 and not ab24921. Again, if you decide to purchase this pair for use in sandwich ELISA they will be covered by our Abpromise.

    I was able to find the conversion factor for IU to SI for TSH, that factor is 1.0 and may be found at the following website:

    http://www.questdiagnostics.com/hcp/intguide/SI_Units.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Our antibody ab24921 does not react with LH, FSH or HCG, however it is unclear which subunit of the TSH protein this antibody is directed against. On the other hand we do know that ab6069 is specific to the beta subunit of TSH but there is no further information at this time if the antibody shows any cross-reactivity with LH, FSH or HCG.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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