Overview

  • Product name
    Anti-TSH antibody [7G12] (HRP)
    See all TSH primary antibodies
  • Description
    Mouse monoclonal [7G12] to TSH (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Specificity
    This antibody specifically recognizes the whole molecule of TSH. It does not cross-react with human LH, FSH, HCG.
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) from human pituitary gland.

  • General notes
    This antibody (conjugate) is ELISA paired with ab6069 (capture).

Properties

Applications

Our Abpromise guarantee covers the use of ab6070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
  • Application notes
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [11E4] to TSH (ab6069).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • CG ALPHA antibody
      • CGA antibody
      • Chorionic gonadotropin alpha polypeptide antibody
      • FSHA antibody
      • Glycoprotein hormones alpha polypeptide antibody
      • GPHA1 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyrotropin beta subunit antibody
      • TSH beta antibody
      • TSHA antibody
      • TSHB antibody
      see all

    References

    This product has been referenced in:
    • Zhou Y  et al. Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide. Int J Mol Med 36:43-52 (2015). WB . Read more (PubMed: 25955519) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA

    Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

    In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

    The suggested secondary antibodies are:
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

    Testing Discount Codes:

    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in sELISA, mouse, rat
    DISCOUNT CODE: xxx-test in sELISA, mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat

    DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
    DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please let me know if there is anything else I can help you with.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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