• Product name
    Anti-TSH Receptor/TSH-R antibody [4C1]
    See all TSH Receptor/TSH-R primary antibodies
  • Description
    Mouse monoclonal [4C1] to TSH Receptor/TSH-R
  • Host species
  • Specificity
    Ab27974 recognises TSH receptor/TSH-R.
  • Tested applications
    Suitable for: IP, Flow Cyt, IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: ELISA
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TSH Receptor/TSH-R. Specific to the external domain.

  • Epitope
    Ab27974 reacts with an epitope located in the extracellular region of TSH receptor.
  • Positive control
    • Human Thyroid gland.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab27974 was purified by affinity chromatography, from tissue culture supernatant.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab27974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/1000.

Use 10µl of the suggested working dilution for 106 cells.




ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 87 kDa.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not required.
  • Application notes
    Is unsuitable for ELISA.
  • Target

    • Function
      Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
    • Tissue specificity
      Expressed in the thyroid.
    • Involvement in disease
      Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
      Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
      Hypothyroidism, congenital, non-goitrous, 1
      Familial gestational hyperthyroidism
      Hyperthyroidism, non-autoimmune
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
      Contains 7 LRR (leucine-rich) repeats.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHNG1 antibody
      • hTSHR I antibody
      • hTSHRI antibody
      • LGR 3 antibody
      • LGR3 antibody
      • MGC75129 antibody
      • Seven transmembrane helix receptor antibody
      • Thyroid adenoma hyperfunctioning antibody
      • Thyroid carcinoma with thyrotoxicosis antibody
      • Thyroid stimulating hormone receptor antibody
      • Thyroid stimulating hormone receptor, isoform 2 antibody
      • Thyroid-stimulating hormone receptor antibody
      • Thyrotropin receptor antibody
      • Thyrotropin receptor I antibody
      • Thyrotropin receptor I, hTSHR I antibody
      • TSH R antibody
      • TSH Receptor antibody
      • TSH-R antibody
      • TSHR antibody
      • TSHR_HUMAN antibody
      see all


    • ab27974 staining TSH Receptor/TSH-R in Human thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).


    This product has been referenced in:
    • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
    • Liu T  et al. Downregulated expression of TSHR is associated with distant metastasis in thyroid cancer. Oncol Lett 14:7506-7512 (2017). Read more (PubMed: 29344196) »
    See all 5 Publications for this product

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