Key features and details
- Rabbit polyclonal to TSH Receptor/TSH-R
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TSH Receptor/TSH-R antibody
See all TSH Receptor/TSH-R primary antibodies
DescriptionRabbit polyclonal to TSH Receptor/TSH-R
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Raji cell lysate; Rat brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20 - 1/100.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
FunctionReceptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Tissue specificityExpressed in the thyroid.
Involvement in diseaseDefects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
Hypothyroidism, congenital, non-goitrous, 1
Familial gestational hyperthyroidism
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 7 LRR (leucine-rich) repeats.
Cellular localizationCell membrane.
- Information by UniProt
- CHNG1 antibody
- hTSHR I antibody
- hTSHRI antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling TSH Receptor/TSH-R with ab214363 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling TSH Receptor/TSH-R with ab214363 at 1/200 dilution overnight at 4°C. Followed by Goat anti-rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C
Flow cytometric analysis of Human Raji cell lysate labeling TSH Receptor/TSH-R with ab214363 at 1/50 dilution for 40 minutes followed by a FITC conjugated secondary (green) compared to negative control cells (blue).
ab214363 has not yet been referenced specifically in any publications.