Product nameAnti-TSH Receptor/TSH-R antibody
See all TSH Receptor/TSH-R primary antibodies
DescriptionRabbit polyclonal to TSH Receptor/TSH-R
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Horse, Cow, Cat, Dog, African green monkey
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay, 1:312500.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 87 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionReceptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Tissue specificityExpressed in the thyroid.
Involvement in diseaseDefects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
Hypothyroidism, congenital, non-goitrous, 1
Familial gestational hyperthyroidism
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 7 LRR (leucine-rich) repeats.
Cellular localizationCell membrane.
- Information by UniProt
- CHNG1 antibody
- hTSHR I antibody
- hTSHRI antibody
Anti-TSH Receptor/TSH-R antibody (ab58917) + Human Jurkat
Predicted band size: 87 kDa
Additional bands at: 21 kDa (possible isoform)
All lanes : Anti-TSH Receptor/TSH-R antibody (ab58917) at 2.5 µg/ml
All lanes : Jurkat
Lysates/proteins at 25 µg per lane.
Predicted band size: 87 kDa
Additional bands at: 21 kDa (possible isoform), 48 kDa (possible isoform)
Gel Concentration: 12%
ab58917 has not yet been referenced specifically in any publications.