Recombinant
RabMAb

Recombinant Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)

Overview

  • Product name

    Anti-TSH Receptor/TSH-R antibody [EPR19751]
    See all TSH Receptor/TSH-R primary antibodies
  • Description

    Rabbit monoclonal [EPR19751] to TSH Receptor/TSH-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TSH Receptor/TSH-R aa 1-450. The exact sequence is proprietary.
    Database link: P16473

  • Positive control

    • IHC-P: Human thyroid, follicular thyroid adenocarcinoma and papillary thyroid carcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19751
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab218108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
  • Tissue specificity

    Expressed in the thyroid.
  • Involvement in disease

    Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
    Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
    Hypothyroidism, congenital, non-goitrous, 1
    Familial gestational hyperthyroidism
    Hyperthyroidism, non-autoimmune
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 7 LRR (leucine-rich) repeats.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHNG1 antibody
    • hTSHR I antibody
    • hTSHRI antibody
    • LGR 3 antibody
    • LGR3 antibody
    • MGC75129 antibody
    • Seven transmembrane helix receptor antibody
    • Thyroid adenoma hyperfunctioning antibody
    • Thyroid carcinoma with thyrotoxicosis antibody
    • Thyroid stimulating hormone receptor antibody
    • Thyroid stimulating hormone receptor, isoform 2 antibody
    • Thyroid-stimulating hormone receptor antibody
    • Thyrotropin receptor antibody
    • Thyrotropin receptor I antibody
    • Thyrotropin receptor I, hTSHR I antibody
    • TSH R antibody
    • TSH Receptor antibody
    • TSH-R antibody
    • TSHR antibody
    • TSHR_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on human normal thyroid is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human follicular thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on follicular thyroid adenocarcinoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human papillary thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on papillary thyroid carcinoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative staining on human normal colon [PMID: 22289392].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative staining on gastric cancer.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab218108 has not yet been referenced specifically in any publications.

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