Key features and details
- Mouse monoclonal [SPM222] to TSH Receptor/TSH-R
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-TSH Receptor/TSH-R antibody [SPM222]
See all TSH Receptor/TSH-R primary antibodies
DescriptionMouse monoclonal [SPM222] to TSH Receptor/TSH-R
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TSH Receptor/TSH-R. (B-Chain protein).
Database link: P16473
- Human thyroid carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Purification notesab218329 was purified from Bioreactor Concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab218329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
FunctionReceptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Tissue specificityExpressed in the thyroid.
Involvement in diseaseDefects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
Hypothyroidism, congenital, non-goitrous, 1
Familial gestational hyperthyroidism
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 7 LRR (leucine-rich) repeats.
Cellular localizationCell membrane.
- Information by UniProt
- CHNG1 antibody
- hTSHR I antibody
- hTSHRI antibody
ab218329 has not yet been referenced specifically in any publications.