Overview

  • Product name

    Anti-TSH Receptor/TSH-R antibody [SPM223]
    See all TSH Receptor/TSH-R primary antibodies
  • Description

    Mouse monoclonal [SPM223] to TSH Receptor/TSH-R
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TSH Receptor/TSH-R. (A-Chain protein).
    Database link: P16473

  • Positive control

    • Human thyroid carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab218328 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SPM223
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab218328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 minutes).

Target

  • Function

    Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
  • Tissue specificity

    Expressed in the thyroid.
  • Involvement in disease

    Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
    Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
    Hypothyroidism, congenital, non-goitrous, 1
    Familial gestational hyperthyroidism
    Hyperthyroidism, non-autoimmune
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 7 LRR (leucine-rich) repeats.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHNG1 antibody
    • hTSHR I antibody
    • hTSHRI antibody
    • LGR 3 antibody
    • LGR3 antibody
    • MGC75129 antibody
    • Seven transmembrane helix receptor antibody
    • Thyroid adenoma hyperfunctioning antibody
    • Thyroid carcinoma with thyrotoxicosis antibody
    • Thyroid stimulating hormone receptor antibody
    • Thyroid stimulating hormone receptor, isoform 2 antibody
    • Thyroid-stimulating hormone receptor antibody
    • Thyrotropin receptor antibody
    • Thyrotropin receptor I antibody
    • Thyrotropin receptor I, hTSHR I antibody
    • TSH R antibody
    • TSH Receptor antibody
    • TSH-R antibody
    • TSHR antibody
    • TSHR_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human thyroid carcinoma tissue labeling TSH Receptor/TSH-R with ab218328 at 2 µg/ml.

References

ab218328 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218328.
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