Overview

  • Product name

  • Description

    Rabbit polyclonal to TSHZ1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 16 amino acids near the N terminal of Human TSHZ1 (NP_005777).

  • Positive control

    • A 20 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab140196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 118 kDa.
ICC Use a concentration of 2.5 µg/ml.

Target

  • Relevance

    This gene (TSHZ1) encodes a colon cancer antigen which was defined by serological analysis of recombinant cDNA expression libraries. The encoded protein is a member of the teashirt C2H2-type zinc-finger protein family and may be involved in transcriptional regulation of developmental processes.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Antigen NY CO 33 antibody
    • NY CO 33 antibody
    • NYCO33 antibody
    • SDCCAG33 antibody
    • Serologically defined colon cancer antigen 33 antibody
    • Teashirt family zinc finger 1 antibody
    • Teashirt homolog 1 antibody
    • Teashirt zinc finger 1 antibody
    • Teashirt zinc finger homeobox 1 antibody
    • TSH1 antibody
    see all

Images

  • Immunofluorescence of TSHZ1 in A20 cells with ab140196 at 20 ug/mL.
  • All lanes : Anti-TSHZ1 antibody (ab140196) at 1 µg/ml

    Lane 1 : A 20 cell lysate
    Lane 2 : A 20 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 118 kDa

  • Immunocytochemical analysis of A20 cells labelling TSHZ1 with ab140196 at 2.5 µg/ml.

References

ab140196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Age P0 brain (Subventricular zone))
Permeabilization
Yes - 0.05% Triton
Specification
Age P0 brain (Subventricular zone)
Blocking step
TNB Blocking Buffer from Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 23 2019

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