Overview

  • Product name

  • Description

    Rabbit polyclonal to TSLP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TSLP (internal sequence). The exact sequence is proprietary. 19 amino acids near the centre of human TSLP. NP_149024.1.
    Database link: Q969D9

  • Positive control

    • Human prostate, brain cortex and liver tissues; Human brain cells; Mouse A20 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 18 kDa.

Target

Images

  • Lane 1 : Anti-TSLP antibody (ab188766) at 0.5 µg/ml
    Lane 2 : Anti-TSLP antibody (ab188766) at 1 µg/ml
    Lane 3 : Anti-TSLP antibody (ab188766) at 2 µg/ml

    All lanes : A20 cell lysate

    Predicted band size: 18 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling TSLP with ab188766 at 5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of Human Brain cells labeling TSLP with ab188766 at 20µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling TSLP with ab188766 at 5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling TSLP with ab188766 at 5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:

  • Dai C  et al. Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection. Exp Eye Res 182:19-29 (2019). Read more (PubMed: 30853520) »
  • Meng Y  et al. Paeonol inhibits the development of 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis via mast and T cells in BALB/c mice. Mol Med Rep 19:3217-3229 (2019). Read more (PubMed: 30816506) »
See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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