Overview

  • Product name
  • Description
    Rabbit polyclonal to TSLP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length TSLP protein (Human),>98% pure

Properties

  • Form
    Lyophilised:Reconstitute with 100ul of sterile water to 1mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 0.5xPBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified by affinity chromatography employing immobilized hTSLP matrix.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.125 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 0.5 - 2 µg/ml. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.
  • Tissue specificity
    Expressed in a number of tissues including heart, liver and prostate.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Thymic stromal lymphopoietin antibody
    • Thymic stromal lymphopoietin protein TSLP antibody
    • Tslp antibody
    • TSLP protein antibody
    • TSLP_HUMAN antibody
    see all

Images

  • Anti-TSLP antibody (ab47943) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa, 51 kDa, 53 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.
  • ab47943  staining TSLP in human prostate tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of ab47943 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Giulbudagian M  et al. Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels. Theranostics 8:450-463 (2018). Read more (PubMed: 29290820) »
  • Tripathi P  et al. Variation in doses and duration of particulate matter exposure in bronchial epithelial cells results in upregulation of different genes associated with airway disorders. Toxicol In Vitro 51:95-105 (2018). WB . Read more (PubMed: 29753051) »
See all 11 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I have checked with the lab and I can confirm that both ab84247 and ab47943 have been prepared in exactly the same way. They have both been produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTSLP. Anti-Human TSLP specific antibody was then purified by affinity chromatography. The only difference is that ab84247 was then biotinylated.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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