• Product name
  • Description
    Rabbit polyclonal to TSLP
  • Host species
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length TSLP protein (Human),>98% pure


  • Form
    Lyophilised:Reconstitute with 100ul of sterile water to 1mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 0.5xPBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified by affinity chromatography employing immobilized hTSLP matrix.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab47943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.125 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 0.5 - 2 µg/ml. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


  • Function
    Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.
  • Tissue specificity
    Expressed in a number of tissues including heart, liver and prostate.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Thymic stromal lymphopoietin antibody
    • Thymic stromal lymphopoietin protein TSLP antibody
    • Tslp antibody
    • TSLP protein antibody
    • TSLP_HUMAN antibody
    see all


  • Anti-TSLP antibody (ab47943) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa, 51 kDa, 53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes

    The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.
  • ab47943  staining TSLP in human prostate tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of ab47943 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Giulbudagian M  et al. Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels. Theranostics 8:450-463 (2018). Read more (PubMed: 29290820) »
  • Tripathi P  et al. Variation in doses and duration of particulate matter exposure in bronchial epithelial cells results in upregulation of different genes associated with airway disorders. Toxicol In Vitro 51:95-105 (2018). WB . Read more (PubMed: 29753051) »
See all 13 Publications for this product

Customer reviews and Q&As


Thank you for contacting us.

I have checked with the lab and I can confirm that both ab84247 and ab47943 have been prepared in exactly the same way. They have both been produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTSLP. Anti-Human TSLP specific antibody was then purified by affinity chromatography. The only difference is that ab84247 was then biotinylated.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More


Sign up