Key features and details
- Rabbit polyclonal to Tspan-8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Tspan-8 antibody
See all Tspan-8 primary antibodies
DescriptionRabbit polyclonal to Tspan-8
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Tspan-8 aa 110-160. The exact sequence is proprietary. (Derived from around Ser129).
Database link: P19075
- IHC-P: Human colon tissue. WB: A549, NIH/3T3 and PC-12 whole cell lysates.
Protein previously labeled as TM4SF3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
Tissue specificityGastric, colon, rectal, and pancreatic carcinomas.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- anti TSPAN8 antibody
- CO029 antibody
- Tetraspanin 8 antibody
Formalin-fixed, paraffin-embedded human colon tissue stained for Tspan-8 using ab230448 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-Tspan-8 antibody (ab230448) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : PC12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 26 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230448 has been referenced in 1 publication.
- Solé-Boldo L et al. Single-cell transcriptomes of the human skin reveal age-related loss of fibroblast priming. Commun Biol 3:188 (2020). PubMed: 32327715