Overview

  • Product name

    Anti-TSPAN17 antibody [EPR9855(2)]
    See all TSPAN17 primary antibodies
  • Description

    Rabbit monoclonal [EPR9855(2)] to TSPAN17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TSPAN17 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96FV3

  • Positive control

    • HepG2, Jurkat, CaCo-2 and SW480 cell lysates; Human Transitional cell bladder carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/50 - 1/100.
IP 1/60.

Target

Images

  • Western blot analysis of Jurkat cell lysate immuoprecipitated with ab180601 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L) peroxidase conjugated secondary antibody used at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human Transitional cell bladder carcinoma tissue labeling TSPAN17 with ab180601 at 1/100 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-TSPAN17 antibody [EPR9855(2)] (ab180601) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : SW480cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

References

ab180601 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180601.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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