Product nameAnti-TSPAN17 antibody [EPR9855(2)]
See all TSPAN17 primary antibodies
DescriptionRabbit monoclonal [EPR9855(2)] to TSPAN17
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TSPAN17 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q96FV3
- HepG2, Jurkat, CaCo-2 and SW480 cell lysates; Human Transitional cell bladder carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||1/50 - 1/100.|
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- F box only protein 23, transmembrane 4 superfamily member 17 antibody
- F box protein FBX23 antibody
- F-box only protein 23 antibody
Western blot analysis of Jurkat cell lysate immuoprecipitated with ab180601 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L) peroxidase conjugated secondary antibody used at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human Transitional cell bladder carcinoma tissue labeling TSPAN17 with ab180601 at 1/100 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-TSPAN17 antibody [EPR9855(2)] (ab180601) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : SW480cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab180601 has not yet been referenced specifically in any publications.