Anti-TSPAN5 antibody (ab236881)
Key features and details
- Rabbit polyclonal to TSPAN5
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TSPAN5 antibody -
Description
Rabbit polyclonal to TSPAN5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human TSPAN5 aa 100-250 (extracellular).
Database link: P62079 -
Positive control
- WB: Jurkat and A549 whole cell lysate. ICC/IF: MCF7 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
---|
WB
1/1000 - 1/5000. |
ICC/IF
1/50 - 1/200. |
Target
-
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 538668 Cow
- Entrez Gene: 10098 Human
- Entrez Gene: 56224 Mouse
- Entrez Gene: 362048 Rat
- Omim: 613136 Human
- SwissProt: Q17QJ5 Cow
- SwissProt: P62079 Human
- SwissProt: P62080 Mouse
see all -
Alternative names
- 2810455A09Rik antibody
- 4930505M03Rik antibody
- AU024142 antibody
see all
Images
-
All lanes : Anti-TSPAN5 antibody (ab236881) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution -
MCF7 (Human breast adenocarcinoma cell line) cells stained for TSPAN5 (Green) using ab236881 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab236881 has been referenced in 1 publication.
- Zheng W et al. MiR-322-5p Alleviates Cell Injury and Impairment of Cognitive Function in Vascular Dementia by Targeting TSPAN5. Yonsei Med J 63:282-291 (2022). PubMed: 35184431