Overview

  • Product name
    Anti-TSPEAR antibody [EPR15505]
  • Description
    Rabbit monoclonal [EPR15505] to TSPEAR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TSPEAR aa 400-500. The exact sequence is proprietary.
    Database link: Q8WU66

  • Positive control
    • K562, C6, RAW264.7 and 293 cell lysate. Human testis lysate. Human kidney and stomach tissue. A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15505
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab192023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 75 kDa).
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

Images

  • All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

  • Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/5000 dilution + human testis lysate at 1/10 dilution

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

  • Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain: ab7291 and ab150120 both at 1/500 dilution. The negative controls: Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.

References

This product has been referenced in:
  • Peled A  et al. Mutations in TSPEAR, Encoding a Regulator of Notch Signaling, Affect Tooth and Hair Follicle Morphogenesis. PLoS Genet 12:e1006369 (2016). ICC/IF ; Human . Read more (PubMed: 27736875) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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