Key features and details
- Rabbit polyclonal to TSPY1/TSPY - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TSPY1/TSPY antibody - C-terminal
See all TSPY1/TSPY primary antibodies
DescriptionRabbit polyclonal to TSPY1/TSPY - C-terminal
At least three isoforms of TSPY1/TSPY are known to exist; ab189383 will detect only TSPY1S.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human TSPY1/TSPY (C terminal). 13 amino acids long, near the C-terminus. NP_003299.2.
Database link: Q01534
- Human testis tissue; A20 cell lysate.
This product was previously labelled as TSPY1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMay be involved in sperm differentiation and proliferation.
Tissue specificitySpecifically expressed in testicular tissues. Isoform 1 and isoform 2 are expressed in spermatogonia and spermatocytes. Found in early testicular carcinoma in situ, spermatogonial cells in testicular tissues of 46,X,Y female and in prostate cancer cell lines.
Involvement in diseaseNote=TSPY is located in the gonadoblastoma critical region and is preferentially expressed in tumor germ cells of gonadoblastoma specimens. Expression also correlates with testicular seminoma and tumorigenesis of the prostate gland.
Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
Developmental stageDetected in 22-week old testis.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Also found in nucleus.
- Information by UniProt
- Cancer/testis antigen 78 antibody
- CT78 antibody
- DYS14 antibody
All lanes : Anti-TSPY1/TSPY antibody - C-terminal (ab189383) at 1 µg/ml
Lane 1 : A20 cell lysate
Lane 2 : A20 cell lysate plus blocking peptide
Predicted band size: 35 kDa
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue sections labeling TSPY1/TSPY using ab189383 at 5 µg/ml.
ab189383 has been referenced in 1 publication.
- Li S et al. Over-expressed Testis-specific Protein Y-encoded 1 as a novel biomarker for male hepatocellular carcinoma. PLoS One 9:e89219 (2014). Human . PubMed: 24586606