Overview

  • Product name

  • Description

    Rabbit polyclonal to TSR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human TSR1 aa 25-75. The exact sequence is proprietary.
    Database link: Q2NL82

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to TSR1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    TSR1 belongs to the BMS1/TSR1 family and TSR1 subfamily. TSR1 is required during maturation of the 40S ribosomal subunit in the nucleolus.
  • Cellular localization

    Nucleus › nucleolus
  • Database links

  • Alternative names

    • AU040765 antibody
    • AW550801 antibody
    • FLJ10534 antibody
    • KIAA1401 antibody
    • MGC131829 antibody
    • mKIAA1401 antibody
    • OTTMUSP00000006478 antibody
    • Pre-rRNA-processing protein TSR1 homolog antibody
    • RP23-174M12.4 antibody
    • TSR1 ribosome maturation factor antibody
    • TSR1, 20S rRNA accumulation, homolog (S. cerevisiae) antibody
    see all

Images

  • All lanes : Anti-TSR1 antibody (ab241604) at 0.4 µg

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 92 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • TSR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241604 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241604 at 1 µg/ml.

    Lane 1: ab241604 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

References

ab241604 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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