Recombinant Anti-TSSK2 antibody [EPR16116] (ab192026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16116] to TSSK2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TSSK2 antibody [EPR16116]
See all TSSK2 primary antibodies -
Description
Rabbit monoclonal [EPR16116] to TSSK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, mouse and rat testis lysates; Human testis tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IHC-P |
1/400 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/400 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at 'Ser-288' and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body. -
Tissue specificity
Testis-specific. Present in mature spermatozoa (at protein level). -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome > centriole. Present in the cytoplasm of elongating spermatids. In spermatozoa, localizes in the equatorial segment, neck, the midpiece and in a specific sperm head compartment (By similarity). In spermatids, concentrates in centrioles during flagellogenesis. - Information by UniProt
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Database links
- Entrez Gene: 23617 Human
- Entrez Gene: 22115 Mouse
- Entrez Gene: 304181 Rat
- Omim: 610710 Human
- SwissProt: Q96PF2 Human
- SwissProt: O54863 Mouse
- Unigene: 694070 Human
- Unigene: 310201 Mouse
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Alternative names
- DGS-G antibody
- DGSG antibody
- DiGeorge syndrome protein G antibody
see all
Images
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Anti-TSSK2 antibody [EPR16116] (ab192026) at 1/1000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-TSSK2 antibody [EPR16116] (ab192026) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TSSK2 with ab192026 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab192026 has not yet been referenced specifically in any publications.