Recombinant
Anti-TST antibody [EPR11646(B)] (ab166625)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TST antibody [EPR11646(B)]
See all TST primary antibodies -
DescriptionRabbit monoclonal [EPR11646(B)] to TST
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human TST was used as an immunogen (UniProt ID: Q16762). (The amino acid sequence is considered to be commercially sensitive).
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Positive control
- Fetal liver, HepG2 and HeLa cell lysates; Human colon tissue and Human liver tissue; HeLa cells; Immunoprecipitation pellet from fetal liver lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11646(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 33 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt.
Target
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FunctionFormation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity.
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Sequence similaritiesContains 2 rhodanese domains.
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DomainThe structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different.
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Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 7263 Human
- Entrez Gene: 22117 Mouse
- Entrez Gene: 25274 Rat
- Omim: 180370 Human
- SwissProt: Q16762 Human
- SwissProt: P52196 Mouse
- SwissProt: P24329 Rat
- Unigene: 474783 Human
see all -
Alternative names
- GlpE antibody
- RDS antibody
- Rhodanese antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/products/166/ab166625/Images/ab166625-9-YCAR11646B-TST-thyroid-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)ab166625 showing +ve staining in Human thyroid gland carcinoma. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/products/166/ab166625/Images/ab166625-8-YCAR11646B-TST-prostate.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)ab166625 showing +ve staining in Human normal prostate. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/products/166/ab166625/Images/ab166625-7-YCAR11646B-TST-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)ab166625 showing +ve staining in Human normal kidney. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/products/166/ab166625/Images/ab166625-6-YCAR11646B-TST-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)ab166625 showing +ve staining in Human breast carcinoma. -
![Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/datasheet/images/166/ab166625/TST-Primary-antibodies-ab166625-1.jpg)
Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)All lanes : Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/datasheet/images/166/ab166625/TST-Primary-antibodies-ab166625-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/datasheet/images/166/ab166625/TST-Primary-antibodies-ab166625-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/datasheet/images/166/ab166625/TST-Primary-antibodies-ab166625-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution. -
![Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)](https://www.abcam.com/ps/datasheet/images/166/ab166625/TST-Primary-antibodies-ab166625-10.jpg)
Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution + immunoprecipitation pellet from fetal liver lysate at 10 µg
Predicted band size: 33 kDa
Protocols
References
This product has been referenced in:
- Zhang S et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). Read more (PubMed: 29262657) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"