Recombinant
RabMAb

Recombinant Anti-TST antibody [EPR11646(B)] (ab166625)

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Overview

  • Product name

    Anti-TST antibody [EPR11646(B)]
    See all TST primary antibodies

  • Description

    Rabbit monoclonal [EPR11646(B)] to TST

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human TST was used as an immunogen (UniProt ID: Q16762). (The amino acid sequence is considered to be commercially sensitive).

  • Positive control

    • Fetal liver, HepG2 and HeLa whole cell lysate (ab150035); Human colon tissue and Human liver tissue; HeLa cells; Immunoprecipitation pellet from fetal liver lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity.

    • Sequence similarities

      Contains 2 rhodanese domains.

    • Domain

      The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • GlpE antibody
      • RDS antibody
      • Rhodanese antibody
      • Thiosulfate sulfurtransferase antibody
      • Thiosulfate sulfurtransferase GlpE antibody
      • THTR_HUMAN antibody
      • TST antibody
      see all

    Images

    • Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      All lanes : Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human thyroid gland carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human normal prostate.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human breast carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution.
    • Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution + immunoprecipitation pellet from fetal liver lysate at 10 µg

      Predicted band size: 33 kDa

    References

    This product has been referenced in:

    • Zhang S  et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). Read more (PubMed: 29262657) »
    See 1 Publication for this product

    Publishing research using ab166625? Please let us know so that we can cite the reference in this datasheet.

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