RecombinantRabMAb

Recombinant Anti-TST antibody [EPR11646(B)] (ab166625)

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Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
  • Anti-TST antibody [EPR11646(B)] (ab166625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11646(B)] to TST
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TST antibody [EPR11646(B)]
    See all TST primary antibodies

  • Description

    Rabbit monoclonal [EPR11646(B)] to TST

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human TST was used as an immunogen (UniProt ID: Q16762). (The amino acid sequence is considered to be commercially sensitive).

  • Positive control

    • Fetal liver, HepG2 and HeLa whole cell lysate (ab150035); Human colon tissue and Human liver tissue; HeLa cells; Immunoprecipitation pellet from fetal liver lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab166625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity.

    • Sequence similarities

      Contains 2 rhodanese domains.

    • Domain

      The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • GlpE antibody
      • RDS antibody
      • Rhodanese antibody
      • Thiosulfate sulfurtransferase antibody
      • Thiosulfate sulfurtransferase GlpE antibody
      • THTR_HUMAN antibody
      • TST antibody
      see all

    Images

    • Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      All lanes : Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human thyroid gland carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human normal prostate.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      ab166625 showing +ve staining in Human breast carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
      Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution.
    • Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
      Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution + immunoprecipitation pellet from fetal liver lysate at 10 µg

      Predicted band size: 33 kDa

    • Anti-TST antibody [EPR11646(B)] (ab166625)
      Anti-TST antibody [EPR11646(B)] (ab166625)

    References (2)

    Publishing research using ab166625? Please let us know so that we can cite the reference in this datasheet.

    ab166625 has been referenced in 2 publications.

    • Zhang Y  et al. Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions. Mol Cell 72:1021-1034.e4 (2018). PubMed: 30472193
    • Zhang S  et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). PubMed: 29262657

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