ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Serine/threonine kinase which is able to phosphorylate tau on serines.
Involvement in disease
Defects in TTBK2 are the cause of spinocerebellar ataxia type 11 (SCA11) [MIM:604432]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA11 is an autosomal dominant cerebellar ataxia (ADCA). It is a relatively benign, late-onset, slowly progressive neurologic disorder.
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Flow cytometric analysis of K562 cells labeling TTBK2 using ab170799 at a 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used.
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human lung tissue labeling TTBK2 with ab170799 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-TTBK2 antibody - N-terminal (ab170799)
Anti-TTBK2 antibody - N-terminal (ab170799) at 1/100 dilution + K562 cell lysate at 35 µg