Overview

  • Product name

  • Description

    Mouse polyclonal to TTC1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human TTC1 protein (NP_003305.1).

  • Positive control

    • Jurkat cell lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Anti-TTC1 antibody (ab88895) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size: 34 kDa
    Observed band size: ~36 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-TTC1 antibody (ab88895) at 1 µg/ml

    Lane 1 : cell lysate from TTC1 transfected 293T cell lysate
    Lane 2 : cell lysate from non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: ~36 kDa why is the actual band size different from the predicted?

  • ab88895 at 10 µg/ml staining TTC1 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluoresence.

References

ab88895 has not yet been referenced specifically in any publications.

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