ab42497 (3µg/ml) staining of paraffin embedded Human Kidney. The tissue sections were subjected to antigen retrieval by microwave in Tris/EDTA buffer. The HRP-staining procedure was used for detection.
Western blot - Anti-TTC10 antibody (ab42497)
Anti-TTC10 antibody (ab42497) at 0.5 µg/ml + Human kidney lysate at 35 µg
Ab42497 staining TTC10 in HepG2 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Samples were incubated with primary antibody at 10µg/ml for 1 hour. An Alexa Fluor® 488 secondary antibody was used at 2 µg/ml. DAPI was used as a nuclear counterstain. An unimmunized goat IgG (10 µg/ml) was used for negative control.
Immunocytochemistry/ Immunofluorescence - Anti-TTC10 antibody (ab42497)This image is a courtesy of Luke Hofkamp
ab42497 staining TTC10 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.