Key features and details
- Rabbit polyclonal to TTC11/FIS1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTC11/FIS1 antibody
See all TTC11/FIS1 primary antibodies
DescriptionRabbit polyclonal to TTC11/FIS1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human TTC11/FIS1.
- Jurkat cell lysates
Previously labelled as TTC11.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab71498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionPromotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
Sequence similaritiesBelongs to the FIS1 family.
Contains 1 TPR repeat.
DomainThe C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
modificationsUbiquitinated by MARCH5.
Cellular localizationMitochondrion outer membrane. Peroxisome membrane.
- Information by UniProt
- 2010003O14Rik antibody
- CGI 135 antibody
- CGI135 antibody
All lanes : Anti-TTC11/FIS1 antibody (ab71498) at 1 µg/ml
All lanes : Jurkat cell lysates
Predicted band size: 17 kDa
Observed band size: 17 kDa
ICC/IF image of ab71498 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71498, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab71498 has been referenced in 44 publications.
- Ouyang H et al. Mst1-Hippo pathway triggers breast cancer apoptosis via inducing mitochondrial fragmentation in a manner dependent on JNK-Drp1 axis. Onco Targets Ther 12:1147-1159 (2019). PubMed: 30809096
- Hou Y et al. Genetic ablation of TAZ induces HepG2 liver cancer cell apoptosis through activating the CaMKII/MIEF1 signaling pathway. Onco Targets Ther 12:1765-1779 (2019). PubMed: 30881030
- Chen S et al. Neuroprotection of hydroxysafflor yellow A in experimental cerebral ischemia/reperfusion injury via metabolic inhibition of phenylalanine and mitochondrial biogenesis. Mol Med Rep 19:3009-3020 (2019). PubMed: 30816517
- Zhang L et al. Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/ß-catenin pathway. Biomed Pharmacother 114:108825 (2019). PubMed: 30981110
- Yao W et al. Large tumor suppressor kinase 2 overexpression attenuates 5-FU-resistance in colorectal cancer via activating the JNK-MIEF1-mitochondrial division pathway. Cancer Cell Int 19:97 (2019). PubMed: 31011291