Overview

  • Product name

  • Description

    Rabbit polyclonal to TTC11/FIS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TTC11/FIS1.

  • Positive control

    • Jurkat cell lysates
  • General notes

    Previously labelled as TTC11. 

Properties

Applications

Our Abpromise guarantee covers the use of ab71498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • Sequence similarities

    Belongs to the FIS1 family.
    Contains 1 TPR repeat.
  • Domain

    The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
  • Post-translational
    modifications

    Ubiquitinated by MARCH5.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010003O14Rik antibody
    • CGI 135 antibody
    • CGI135 antibody
    • FIS 1 antibody
    • FIS1 antibody
    • Fis1 homolog antibody
    • FIS1, S. cerevisiae, homolog of antibody
    • FIS1_HUMAN antibody
    • Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody
    • Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody
    • Fission 1 (mitochondrial outer membrane) homolog antibody
    • Fission 1 homolog antibody
    • H NH0132A01.6 antibody
    • hFis 1 antibody
    • hFis1 antibody
    • Mitochondrial fission 1 protein antibody
    • mitochondrial fission molecule antibody
    • Tetratricopeptide repeat domain 11 antibody
    • Tetratricopeptide repeat protein 11 antibody
    • TPR repeat protein 11 antibody
    • TTC 11 antibody
    • TTC11 antibody
    see all

Images

  • All lanes : Anti-TTC11/FIS1 antibody (ab71498) at 1 µg/ml

    All lanes : Jurkat cell lysates

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • ICC/IF image of ab71498 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71498, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ouyang H  et al. Mst1-Hippo pathway triggers breast cancer apoptosis via inducing mitochondrial fragmentation in a manner dependent on JNK-Drp1 axis. Onco Targets Ther 12:1147-1159 (2019). Read more (PubMed: 30809096) »
  • Hou Y  et al. Genetic ablation of TAZ induces HepG2 liver cancer cell apoptosis through activating the CaMKII/MIEF1 signaling pathway. Onco Targets Ther 12:1765-1779 (2019). Read more (PubMed: 30881030) »
See all 31 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 09 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
20 µg
Specification
Primary Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 15 2013

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