Product nameAnti-TTC11/FIS1 antibody [EPR8412]
See all TTC11/FIS1 primary antibodies
DescriptionRabbit monoclonal [EPR8412] to TTC11/FIS1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Cow, Human
Synthetic peptide within Human TTC11/FIS1. The exact sequence is proprietary.
Database link: Q9Y3D6
- Human spleen, Jurkat, 293T and (D) Raji cell lysates; Human kidney tissue and Human ovarian adenocarcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as TTC11.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionPromotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
Sequence similaritiesBelongs to the FIS1 family.
Contains 1 TPR repeat.
DomainThe C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
modificationsUbiquitinated by MARCH5.
Cellular localizationMitochondrion outer membrane. Peroxisome membrane.
- Information by UniProt
- 2010003O14Rik antibody
- CGI 135 antibody
- CGI135 antibody
All lanes : Anti-TTC11/FIS1 antibody [EPR8412] (ab156865) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TTC11/FIS1 using ab156865 at 1/100.
Immunohistochemical analysis of paraffin embedded Human ovarian adenocarcinoma tissue labeling TTC11/FIS1 using ab156865 at 1/100.
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Zhang D et al. Increased mitochondrial fission is critical for hypoxia-induced pancreatic beta cell death. PLoS One 13:e0197266 (2018). Read more (PubMed: 29768513) »
- Kristensen MD et al. Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity. Acta Physiol (Oxf) N/A:e13100 (2018). Read more (PubMed: 29791782) »