Recombinant
RabMAb

Recombinant Anti-TTC11/FIS1 antibody [EPR8412] (Alexa Fluor® 488) (ab225220)

Overview

  • Product name

    Anti-TTC11/FIS1 antibody [EPR8412] (Alexa Fluor® 488)
    See all TTC11/FIS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8412] to TTC11/FIS1 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TTC11/FIS1. The exact sequence is proprietary.
    Database link: Q9Y3D6

  • Positive control

    • ICC/IF: Ramos cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in Ramos cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • Sequence similarities

    Belongs to the FIS1 family.
    Contains 1 TPR repeat.
  • Domain

    The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
  • Post-translational
    modifications

    Ubiquitinated by MARCH5.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010003O14Rik antibody
    • CGI 135 antibody
    • CGI135 antibody
    • FIS 1 antibody
    • FIS1 antibody
    • Fis1 homolog antibody
    • FIS1, S. cerevisiae, homolog of antibody
    • FIS1_HUMAN antibody
    • Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody
    • Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody
    • Fission 1 (mitochondrial outer membrane) homolog antibody
    • Fission 1 homolog antibody
    • H NH0132A01.6 antibody
    • hFis 1 antibody
    • hFis1 antibody
    • Mitochondrial fission 1 protein antibody
    • mitochondrial fission molecule antibody
    • Tetratricopeptide repeat domain 11 antibody
    • Tetratricopeptide repeat protein 11 antibody
    • TPR repeat protein 11 antibody
    • TTC 11 antibody
    • TTC11 antibody
    see all

Images

  • ab225220 staining TTC11/FIS1 in Ramos (Human Burkitt's lymphoma cell line) cells.

    The cells were fixed with 4% formaldehyde (10 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab225220 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225220 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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