Product nameAnti-TTC19 antibody
See all TTC19 primary antibodies
DescriptionRabbit polyclonal to TTC19
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- RT 4 and U 251 cell lysates; Human liver and tonsil tissue lysates; Human rectum tissue; U 251 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 42 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
FunctionMitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.
Involvement in diseaseMitochondrial complex III deficiency, nuclear 2
Sequence similaritiesBelongs to the TTC19 family.
Contains 5 TPR repeats.
Cellular localizationMitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others.
- Information by UniProt
- 2010204O13Rik antibody
- FLJ20343 antibody
- MGC138312 antibody
All lanes : Anti-TTC19 antibody (ab204236) at 1/500 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 42 kDa
Immunofluorescent analysis of U 251 cells (PFA-fixed/Triton X-100 permeabilized) labeling TTC19 with ab204236 at 4 µg/ml.
Immunohistochemical analysis of Paraffin-embedded Human rectum tissue labeling TTC19 with ab204236 at 1/200 dilution.
ab204236 has not yet been referenced specifically in any publications.