Key features and details
- Rabbit polyclonal to TTC19
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTC19 antibody
See all TTC19 primary antibodies
DescriptionRabbit polyclonal to TTC19
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- RT 4 and U 251 cell lysates; Human liver and tonsil tissue lysates; Human rectum tissue; U 251 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 42 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionMitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.
Involvement in diseaseMitochondrial complex III deficiency, nuclear 2
Sequence similaritiesBelongs to the TTC19 family.
Contains 5 TPR repeats.
Cellular localizationMitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others.
- Information by UniProt
- 2010204O13Rik antibody
- FLJ20343 antibody
- MGC138312 antibody
All lanes : Anti-TTC19 antibody (ab204236) at 1/500 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 42 kDa
Immunofluorescent analysis of U 251 cells (PFA-fixed/Triton X-100 permeabilized) labeling TTC19 with ab204236 at 4 µg/ml.
Immunohistochemical analysis of Paraffin-embedded Human rectum tissue labeling TTC19 with ab204236 at 1/200 dilution.
ab204236 has not yet been referenced specifically in any publications.