Product nameAnti-TTC19 antibody [EPR13184-63]
See all TTC19 primary antibodies
DescriptionRabbit monoclonal [EPR13184-63] to TTC19
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TTC19 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q6DKK2
- Human ovarian cancer and HT-1080 cell lysates, HT-1080 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).|
|IP||1/20 - 1/40.|
|ICC/IF||1/50 - 1/100.|
FunctionMitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.
Involvement in diseaseMitochondrial complex III deficiency, nuclear 2
Sequence similaritiesBelongs to the TTC19 family.
Contains 5 TPR repeats.
Cellular localizationMitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others.
- Information by UniProt
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All lanes : Anti-TTC19 antibody [EPR13184-63] (ab181191) at 1/5000 dilution
Lane 1 : Human ovarian cancer lysate
Lane 2 : HT-1080 lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Predicted band size: 42 kDa
Additional bands at: 35 kDa (possible mature (processed) protein)
Immunocytochemical analysis of HT-1080 cells fixed in 4% paraformaldehyde labeling TTC19 with ab181191 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counterstained with DAP1 (blue)
Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
ab181191 has not yet been referenced specifically in any publications.