Recombinant Anti-TTC19 antibody [EPR13184-63] (ab181191)
![Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.com/ps/products/181/ab181191/Images/ab181191-214659-WB1.png "Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13184-63] to TTC19
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-TTC19 antibody [EPR13184-63]
See all TTC19 primary antibodies -
Description
Rabbit monoclonal [EPR13184-63] to TTC19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TTC19 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q6DKK2 -
Positive control
- Human ovarian cancer and HT-1080 cell lysates, HT-1080 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13184-63 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab181191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa). | |
| IP | 1/20 - 1/40. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence. -
Involvement in disease
Mitochondrial complex III deficiency, nuclear 2 -
Sequence similarities
Belongs to the TTC19 family.
Contains 5 TPR repeats. -
Cellular localization
Mitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others. - Information by UniProt
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Database links
- Entrez Gene: 54902 Human
- Omim: 613814 Human
- SwissProt: Q6DKK2 Human
- Unigene: 462316 Human
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Alternative names
- 2010204O13Rik antibody
- FLJ20343 antibody
- MGC138312 antibody
see all
Images
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Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)All lanes : Anti-TTC19 antibody [EPR13184-63] (ab181191) at 1/5000 dilution
Lane 1 : Human ovarian cancer lysate
Lane 2 : HT-1080 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 42 kDa
Additional bands at: 35 kDa (possible mature (processed) protein) -
![Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.com/ps/products/181/ab181191/Images/ab181191-214658-icc1.png)
Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (ab181191)Immunocytochemical analysis of HT-1080 cells fixed in 4% paraformaldehyde labeling TTC19 with ab181191 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counterstained with DAP1 (blue)
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![Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.com/ps/products/181/ab181191/Images/ab181191-214657-ip-copy.png)
Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (ab181191)Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
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![Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.com/ps/products/181/ab181191/Images/ab181191-4-benefits-of-recombinant-antibodies.png)
Anti-TTC19 antibody [EPR13184-63] (ab181191)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab181191 has not yet been referenced specifically in any publications.
