Recombinant Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13184] to TTC19 - C-terminal
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TTC19 antibody [EPR13184] - C-terminal
See all TTC19 primary antibodies -
Description
Rabbit monoclonal [EPR13184] to TTC19 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT-1080 cell and Human fetal muscle, fetal heart and ovarian cancer lysates. HT-1080 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13184 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).
The observed MW is lower than the predicted 42kDa, which may be due to the removing of 8KDa transit peptide. |
ICC/IF |
1/100 - 1/250.
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IP |
1/70.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa). The observed MW is lower than the predicted 42kDa, which may be due to the removing of 8KDa transit peptide. |
ICC/IF
1/100 - 1/250. |
IP
1/70. |
Target
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Function
Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence. -
Involvement in disease
Mitochondrial complex III deficiency, nuclear 2 -
Sequence similarities
Belongs to the TTC19 family.
Contains 5 TPR repeats. -
Cellular localization
Mitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others. - Information by UniProt
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Database links
- Entrez Gene: 54902 Human
- Entrez Gene: 72795 Mouse
- Entrez Gene: 691506 Rat
- Omim: 613814 Human
- SwissProt: Q6DKK2 Human
- SwissProt: Q8CC21 Mouse
- SwissProt: D4A6D7 Rat
- Unigene: 462316 Human
see all -
Alternative names
- 2010204O13Rik antibody
- FLJ20343 antibody
- MGC138312 antibody
see all
Images
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All lanes : Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution
Lane 1 : Human fetal muscle tissue lysate
Lane 2 : Human ovarian cancer tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)
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Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181069 has not yet been referenced specifically in any publications.