Product nameAnti-TTC28/TPRBK antibody - C-terminal
DescriptionRabbit polyclonal to TTC28/TPRBK - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
This product was previously labelled as TTC28
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab197076 was affinity purified using an epitope specific to TTC28/TPRBK immobilized on solid support.
Our Abpromise guarantee covers the use of ab197076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 271 kDa.|
Sequence similaritiesContains 28 TPR repeats.
- Information by UniProt
- 2310015L07Rik antibody
- 6030435N04 antibody
- AI428795 antibody
All lanes : Anti-TTC28/TPRBK antibody - C-terminal (ab197076) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 271 kDa
Exposure time: 3 minutes
Lysates prepared wtih NETN lysis buffer.
Detection of TTC28/TPRBK in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197076 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab197076 has not yet been referenced specifically in any publications.