Recombinant Anti-TTC4 antibody [EPR13381-76] (ab181195)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13381-76] to TTC4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TTC4 antibody [EPR13381-76]
See all TTC4 primary antibodies -
Description
Rabbit monoclonal [EPR13381-76] to TTC4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HepG2, MCF7 and U87-MG cell lysates; Human testis and Human transitional cell carcinoma of bladder tissue; MCF7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13381-76 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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ICC/IF |
1/100 - 1/250.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
1/100 - 1/250. |
Target
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Relevance
TTC4 contains three tetratricopeptide repeats. The 34-amino acid tetratricopeptide repeat (TPR) motif is found in a variety of proteins and may mediate protein-protein or protein-membrane interactions. -
Database links
- Entrez Gene: 7268 Human
- Entrez Gene: 72354 Mouse
- Omim: 606753 Human
- SwissProt: O95801 Human
- SwissProt: Q8R3H9 Mouse
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Alternative names
- DKFZp781B0622 antibody
- FLJ41930 antibody
- MGC5097 antibody
see all
Images
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All lanes : Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181195 has been referenced in 2 publications.
- He X et al. Promoting TTC4 and HSP70 interaction and translocation of annexin A7 to lysosome inhibits apoptosis in vascular endothelial cells. FASEB J 34:12932-12945 (2020). PubMed: 33000523
- He X et al. MROH7-TTC4 read-through lncRNA suppresses vascular endothelial cell apoptosis and is upregulated by inhibition of ANXA7 GTPase activity. FEBS J 286:4937-4950 (2019). PubMed: 31408583