- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TTC5 antibody
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DescriptionRabbit polyclonal to TTC5
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
A 14 amino acid peptide from near the carboxy terminus of human TTC5.
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Positive control
- RAW264.7 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab36855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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RelevanceTetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.
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Cellular localizationNucleus. Cytoplasm. Note: Phosphorylation at Ser-203 results in nuclear localization, while unphosphorylated protein localizes to the cytoplasm
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Database links
- Entrez Gene: 91875 Human
- Entrez Gene: 219022 Mouse
- SwissProt: Q8N0Z6 Human
- SwissProt: Q99LG4 Mouse
- Unigene: 98553 Human
- Unigene: 276362 Mouse
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Alternative names
- Strap antibody
- Stress-responsive activator of p300 antibody
- Tetratricopeptide repeat domain 5 antibody
- TPR repeat protein 5 antibody
Images
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Western blot - Anti-TTC5 antibody (ab36855)Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/ml
Lane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/ml
Lane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC5 antibody (ab36855)ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.
Protocols
References
This product has been referenced in:
- Davies L et al. Regulation of glucocorticoid receptor activity by a stress responsive transcriptional cofactor. Mol Endocrinol 25:58-71 (2011). WB ; Human . Read more (PubMed: 21147850) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"