Key features and details
- Rabbit polyclonal to TTC5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TTC5 antibody
DescriptionRabbit polyclonal to TTC5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
A 14 amino acid peptide from near the carboxy terminus of human TTC5.
- RAW264.7 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceTetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.
Cellular localizationNucleus. Cytoplasm. Note: Phosphorylation at Ser-203 results in nuclear localization, while unphosphorylated protein localizes to the cytoplasm
- Strap antibody
- Stress-responsive activator of p300 antibody
- Tetratricopeptide repeat domain 5 antibody
- TPR repeat protein 5 antibody
Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/ml
Lane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/ml
Lane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg/ml per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC
ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.
ab36855 has been referenced in 1 publication.
- Davies L et al. Regulation of glucocorticoid receptor activity by a stress responsive transcriptional cofactor. Mol Endocrinol 25:58-71 (2011). WB ; Human . PubMed: 21147850