Overview

  • Product name

  • Description

    Rabbit polyclonal to TTC7A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: TSRHLKGCHP LDYELTYFLE AALQSAYVKN LKKGNIVKGM RELREVLRTV ETKATQNFKV MAAKHLAGVL LHSLSEECYW SPLSHPLPEF MGKE, corresponding to amino acids 222-315 of Human TTC7A

  • Positive control

    • Human stomach tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab122363, at 1/15 dilution, staining TTC7A in paraffin-embedded Human stomach tissue (glandular cells) by immunohistochemistry.

References

ab122363 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for these additional details. I would recommend increasing your incubation with primary antibody to overnight at 4 degrees to get the strongest signal. This is how we routinely test our antibodies for IHC. You may also wish to reduce the antigen retrieval time, or do several time points testing at 20, 30 and 40 minutes to get the optimal retrieval. Please let me know if these suggestions do not improve your results.

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