Overview

  • Product name

  • Description

    Rabbit polyclonal to TTF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TTF1 (internal sequence). The exact sequence is proprietary.
    Database link: Q15361

  • Positive control

    • WB: HEK-293T nuclear and whole cell extracts. IP: HEK-293T whole cell extracts. IHC-P: Human small cell lung cancer tissue.
  • General notes

     This product was previously labelled as Transcription termination factor 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000.

Target

  • Function

    Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
  • Sequence similarities

    Contains 2 Myb-like domains.
  • Domain

    The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • RNA polymerase I termination factor antibody
    • Transcription termination factor 1 antibody
    • Transcription termination factor I antibody
    • Transcription termination factor RNA polymerase I antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF-I antibody
    • Ttf1 antibody
    • TTF1_HUMAN antibody
    see all

Images

  • Paraffin-embedded human small cell lung cancer tissue stained for TTF1 with ab229564 at 1/500 dilution in immunohistochemical analysis.

  • TTF1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab229564. Western blot was performed from the immunoprecipitate.

    Lane 1: HEK-293T whole cell extract (Input).

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab229564 IP in HEK-293T whole cell extract.

  • All lanes : Anti-TTF1 antibody (ab229564) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.


    5% SDS-PAGE

References

ab229564 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229564.
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