Anti-TTF1 antibody (ab87726)
Key features and details
- Rabbit polyclonal to TTF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TTF1 antibody
See all TTF1 primary antibodies -
Description
Rabbit polyclonal to TTF1 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide corresponding to Human TTF1 aa 1-50 (N terminal). (CAA58807.1)
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Positive control
- HeLa or 293T cell lysate
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General notes
This product was previously labelled as Transcription termination factor 1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Chimpanzee
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Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 103 kDa. |
Target
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Function
Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. -
Sequence similarities
Contains 2 Myb-like domains. -
Domain
The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 7270 Human
- Omim: 600777 Human
- SwissProt: Q15361 Human
- Unigene: 54780 Human
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Alternative names
- RNA polymerase I termination factor antibody
- Transcription termination factor 1 antibody
- Transcription termination factor I antibody
see all
Images
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All lanes : Anti-TTF1 antibody (ab87726) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~118 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab87726 has been referenced in 1 publication.
- Komatsu H et al. Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma. Oncol Rep 35:2073-80 (2016). PubMed: 26821084