Anti-TTF1 antibody (ab87726)

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Overview

  • Product name

    Anti-TTF1 antibody
    See all TTF1 primary antibodies

  • Description

    Rabbit polyclonal to TTF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee

  • Immunogen

    Synthetic peptide corresponding to Human TTF1 aa 1-50 (N terminal). (CAA58807.1)

  • Positive control

    • HeLa or 293T cell lysate

  • General notes

     This product was previously labelled as Transcription termination factor 1

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.

Target

  • Function

    Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.

  • Sequence similarities

    Contains 2 Myb-like domains.

  • Domain

    The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    • Alternative names

      • RNA polymerase I termination factor antibody
      • Transcription termination factor 1 antibody
      • Transcription termination factor I antibody
      • Transcription termination factor RNA polymerase I antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF-I antibody
      • Ttf1 antibody
      • TTF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TTF1 antibody (ab87726)
      Western blot - Anti-TTF1 antibody (ab87726)
      All lanes : Anti-TTF1 antibody (ab87726) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique.

      Predicted band size: 103 kDa
      Observed band size: ~118 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds

    References

    This product has been referenced in:

    • Komatsu H  et al. Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma. Oncol Rep 35:2073-80 (2016). Read more (PubMed: 26821084) »
    See 1 Publication for this product

    Publishing research using ab87726? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I would like to know if your TTF-1 Abs are against Transcription
    Termination Factor 1 or Thyroid transcription factor that is encoded by
    the NKX2-1 gene, tehre seems to be a bit of confusion on the Abcam
    reference sheets .

    Read More
    Answer

    Thank you for keeping in touch.

    Please take my apologies for the long delay of my reply. I have contacted the originators of some of our relevant antibodies in order to get a confirmation (mistakes can happen, especially with those similar target names).

    Now I am happy to let you know that all of our TTF1 antibodies are directed against theThyroid transcription factor 1, also known as NKX2-1(SwissProtID P43699, http://www.uniprot.org/uniprot/P43699), as also stated in the Alternative names section on each datasheet.

    The antibodies in our catalogue that recognise the Transcription Termination Factor 1 (SwissProtID Q15361, http://www.uniprot.org/uniprot/Q15361) can be found under this full name, as for example ab87726 (https://www.abcam.com/index.html?datasheet=87726 (or use the following: https://www.abcam.com/index.html?datasheet=87726).).

    Thank you again for checking with us and for your patience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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