Anti-TTF1 antibody (ab87726)

Submit a review Q&A (1)References (1)
Western blot - Anti-TTF1 antibody (ab87726)

    Key features and details

    • Rabbit polyclonal to TTF1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-TTF1 antibody
      See all TTF1 primary antibodies

    • Description

      Rabbit polyclonal to TTF1

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human TTF1 aa 1-50 (N terminal). (CAA58807.1)

    • Positive control

      • HeLa or 293T cell lysate

    • General notes

       This product was previously labelled as Transcription termination factor 1

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.1% BSA, Tris buffered saline
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab87726 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Application Abreviews Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
    Notes
    WB
    1/2000 - 1/10000. Predicted molecular weight: 103 kDa.

    Target

    • Function

      Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.

    • Sequence similarities

      Contains 2 Myb-like domains.

    • Domain

      The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Nucleus > nucleolus.
    • Information by UniProt

    • Database links

      • Alternative names

        • RNA polymerase I termination factor antibody
        • Transcription termination factor 1 antibody
        • Transcription termination factor I antibody
        • Transcription termination factor RNA polymerase I antibody
        • TTF 1 antibody
        • TTF-1 antibody
        • TTF-I antibody
        • Ttf1 antibody
        • TTF1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-TTF1 antibody (ab87726)
        Western blot - Anti-TTF1 antibody (ab87726)
        All lanes : Anti-TTF1 antibody (ab87726) at 0.04 µg/ml

        Lane 1 : Whole cell lysate from HeLa cells at 50 µg
        Lane 2 : Whole cell lysate from HeLa cells at 15 µg
        Lane 3 : Whole cell lysate from HeLa cells at 5 µg
        Lane 4 : Whole cell lysate from 293T cells at 50 µg

        Developed using the ECL technique.

        Predicted band size: 103 kDa
        Observed band size: ~118 kDa why is the actual band size different from the predicted?


        Exposure time: 30 seconds

      References (1)

      Publishing research using ab87726? Please let us know so that we can cite the reference in this datasheet.

      ab87726 has been referenced in 1 publication.

      • Komatsu H  et al. Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma. Oncol Rep 35:2073-80 (2016). PubMed: 26821084

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Question

      I would like to know if your TTF-1 Abs are against Transcription
      Termination Factor 1 or Thyroid transcription factor that is encoded by
      the NKX2-1 gene, tehre seems to be a bit of confusion on the Abcam
      reference sheets .

      Read More
      Answer

      Thank you for keeping in touch.

      Please take my apologies for the long delay of my reply. I have contacted the originators of some of our relevant antibodies in order to get a confirmation (mistakes can happen, especially with those similar target names).

      Now I am happy to let you know that all of our TTF1 antibodies are directed against theThyroid transcription factor 1, also known as NKX2-1(SwissProtID P43699, http://www.uniprot.org/uniprot/P43699), as also stated in the Alternative names section on each datasheet.

      The antibodies in our catalogue that recognise the Transcription Termination Factor 1 (SwissProtID Q15361, http://www.uniprot.org/uniprot/Q15361) can be found under this full name, as for example ab87726 (https://www.abcam.com/TTF1-antibody-ab87726.html (or use the following: https://www.abcam.com/TTF1-antibody-ab87726.html).).

      Thank you again for checking with us and for your patience.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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