Recombinant Anti-TTF1 antibody [EP1584Y] (ab76013)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1584Y] to TTF1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TTF1 antibody [EP1584Y]
See all TTF1 primary antibodies -
Description
Rabbit monoclonal [EP1584Y] to TTF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
-
Positive control
- ICC/IF: Human thyroid cells; Rat bronchi cells. IHC-P: Human lung carcinoma and thyroid carcinoma tissue; Rat lung tissue; Mouse lung tissue. WB: HeLa and HEK-293 cell lysate; Rat and mouse lung lysate. Flow Cyt: HeLa and A549 cells. IHC-Fr: Murine fetal lung tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1584Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab76013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/2000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 38-42 kDa).Can be blocked with TTF1 peptide (ab187893). For unpurified use at 1/500- 1/1000. |
Target
-
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. -
Tissue specificity
Thyroid and lung. -
Involvement in disease
Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7080 Human
- Entrez Gene: 21869 Mouse
- Entrez Gene: 25628 Rat
- Omim: 600635 Human
- SwissProt: P43699 Human
- SwissProt: P50220 Mouse
- SwissProt: P23441 Rat
- Unigene: 94367 Human
see all -
Alternative names
- AV026640 antibody
- BCH antibody
- Benign chorea antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274263-ab76013IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
-
![Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274260-ab76013WB1.jpg)
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/10000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274266-ab76013IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
-
![Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274262-ab76013WB3.jpg)
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)Anti-TTF1 antibody [EP1584Y] (ab76013) (purified) + Rat lung lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274265-ab76013IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
-
![Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274261-ab76013WB2.jpg)
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (purified) + Mouse lung lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/TTF1-Primary-antibodies-ab76013-41.jpg)
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)This image is courtesy of an anonymous AbreviewAnti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (unpurified) + HEK293 whole cell lysate at 30 µg
Secondary
HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked with 5% milk -
![Immunocytochemistry/ Immunofluorescence - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/TTF1-Primary-antibodies-ab76013-42.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TTF1 antibody [EP1584Y] (ab76013)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of rat bronchi cells, staining TTF1 with unpurified ab76013.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)](https://www.abcam.com/ps/products/76/ab76013/Images/ab76013-274264-ab76013IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Protocols
References (69)
ab76013 has been referenced in 69 publications.
- Hou Z et al. Wnt/Fgf crosstalk is required for the specification of basal cells in the mouse trachea. Development 146:N/A (2019). PubMed: 30696710
- Wang GZ et al. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun 10:1125 (2019). PubMed: 30850589
- Riley A et al. A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform. BMC Cancer 19:259 (2019). PubMed: 30902086
- Fukui E et al. Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice. Stem Cells Int 2019:5179172 (2019). PubMed: 31281377
- Saad MI et al. The ADAM17 Protease Promotes Tobacco Smoke Carcinogen-induced Lung Tumourigenesis. Carcinogenesis N/A:N/A (2019). PubMed: 31257400
