Recombinant Anti-TTF1 antibody [EPR8190-138] (ab137061)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8190-138] to TTF1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-TTF1 antibody [EPR8190-138]
See all TTF1 primary antibodies
Description

Rabbit monoclonal [EPR8190-138] to TTF1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human TTF1. The exact sequence is proprietary.
Positive control
- Human fetal lung, Human lung adenocarcinoma, Rat and Mouse lung tissue lysates; Human lung carcinoma and Human papillary thyroid carcinoma, Lung squamous carcinoma, Small cell lung carcinoma, Thyroid gland, Thyroid gland papillary carcinoma and Normal lung tissues
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8190-138
Isotype

IgG
Research areas

Alternative Versions
- Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human TTF1 protein (ab159708)

Our Abpromise guarantee covers the use of ab137061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Application notes

Is unsuitable for ICC.

Function

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
Tissue specificity

Thyroid and lung.
Involvement in disease

Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
Sequence similarities

Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
Post-translational
modifications

Phosphorylated on serine residues.
Cellular localization

Nucleus.
Target information above from: UniProt accession P43699 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7080 Human
- Entrez Gene: 21869 Mouse
- Entrez Gene: 25628 Rat
- Omim: 600635 Human
- SwissProt: P43699 Human
- SwissProt: P50220 Mouse
- SwissProt: P23441 Rat
- Unigene: 94367 Human
- Unigene: 89972 Mouse
- Unigene: 34265 Rat
see all
Alternative names
- AV026640 antibody
- BCH antibody
- Benign chorea antibody
- BHC antibody
- Homeobox protein NK 2 homolog A antibody
- Homeobox protein NK-2 homolog A antibody
- Homeobox protein Nkx 2.1 antibody
- Homeobox protein Nkx-2.1 antibody
- Homeobox protein Nkx2.1 antibody
- NK 2 antibody
- NK 2 homolog A antibody
- NK2 antibody
- NK2 homeobox 1 antibody
- NK2, drosophila, homolog of, A antibody
- NK2.1, mouse, homolog of antibody
- Nkx 2 1 antibody
- NKX 2.1 antibody
- NKX 2A antibody
- NKX2 1 antibody
- Nkx2-1 antibody
- NKX2.1 antibody
- NKX21_HUMAN antibody
- NKX2A antibody
- T EBP antibody
- T/EBP antibody
- TEBP antibody
- Thyroid nuclear factor 1 antibody
- Thyroid nuclear factor antibody
- Thyroid specific enhancer binding protein antibody
- Thyroid transcription factor 1 antibody
- Tin man antibody
- Tinman antibody
- TITF 1 antibody
- TITF1 antibody
- TTF 1 antibody
- TTF-1 antibody
- TTF1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing positive staining in Lung squamous carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing positive staining in Small cell lung carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland papillary carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis using ab137061 showing positive staining in Normal lung tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] (ab137061)

Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TTF1 antibody [EPR8190-138] (ab137061)

All lanes : Anti-TTF1 antibody [EPR8190-138] (ab137061) at 1/1000 dilution

Lane 1 : Human fetal lung tissue lysate
Lane 2 : Human lung adenocarcinoma tissue lysate
Lane 3 : Rat lung tissue lysate
Lane 4 : Mouse lung tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 39 kDa
Anti-TTF1 antibody [EPR8190-138] (ab137061)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet

Publishing research using ab137061? Please let us know so that we can cite the reference in this datasheet.

ab137061 has been referenced in 1 publication.

Huang Q et al. Preclinical Modeling of KIF5B-RET Fusion Lung Adenocarcinoma. Mol Cancer Ther 15:2521-2529 (2016). PubMed: 27496134

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-TTF1 antibody [EPR8190-138] (ab137061)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translational
modifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (1)

Customer reviews and Q&As

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (1)

Customer reviews and Q&As

Post-translational
modifications