Recombinant Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free
See all TTF1 primary antibodies -
Description
Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment.
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Positive control
- Human fetal lung, Human lung adenocarcinoma, Rat and Mouse lung tissue lysates; Human lung carcinoma and Human papillary thyroid carcinoma, Lung squamous carcinoma, Small cell lung carcinoma, Thyroid gland, Thyroid gland papillary carcinoma and Normal lung tissues
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General notes
ab227576 is the carrier-free version of ab137061.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8190-138 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. -
Tissue specificity
Thyroid and lung. -
Involvement in disease
Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7080 Human
- Entrez Gene: 21869 Mouse
- Entrez Gene: 25628 Rat
- Omim: 600635 Human
- SwissProt: P43699 Human
- SwissProt: P50220 Mouse
- SwissProt: P23441 Rat
- Unigene: 94367 Human
see all -
Alternative names
- AV026640 antibody
- BCH antibody
- Benign chorea antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis using ab137061 showing positive staining in Lung squamous carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland papillary carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis using ab137061 showing negative staining in Normal liver tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis using ab137061 showing positive staining in Normal lung tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).
Immunohistochemical analysis using ab137061 showing positive staining in Small cell lung carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).
Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab227576 has been referenced in 1 publication.
- Huang Q et al. Preclinical Modeling of KIF5B-RET Fusion Lung Adenocarcinoma. Mol Cancer Ther 15:2521-2529 (2016). PubMed: 27496134