Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free
    See all TTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment.

  • Positive control

    • Human fetal lung, Human lung adenocarcinoma, Rat and Mouse lung tissue lysates; Human lung carcinoma and Human papillary thyroid carcinoma, Lung squamous carcinoma, Small cell lung carcinoma, Thyroid gland, Thyroid gland papillary carcinoma and Normal lung tissues

  • General notes

    ab227576 is the carrier-free version of ab137061.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8190-138

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
Application notes
Is unsuitable for ICC.

Target

  • Function

    Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.

  • Tissue specificity

    Thyroid and lung.

  • Involvement in disease

    Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
    Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.

  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylated on serine residues.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AV026640 antibody
    • BCH antibody
    • Benign chorea antibody
    • BHC antibody
    • Homeobox protein NK 2 homolog A antibody
    • Homeobox protein NK-2 homolog A antibody
    • Homeobox protein Nkx 2.1 antibody
    • Homeobox protein Nkx-2.1 antibody
    • Homeobox protein Nkx2.1 antibody
    • NK 2 antibody
    • NK 2 homolog A antibody
    • NK2 antibody
    • NK2 homeobox 1 antibody
    • NK2, drosophila, homolog of, A antibody
    • NK2.1, mouse, homolog of antibody
    • Nkx 2 1 antibody
    • NKX 2.1 antibody
    • NKX 2A antibody
    • NKX2 1 antibody
    • Nkx2-1 antibody
    • NKX2.1 antibody
    • NKX21_HUMAN antibody
    • NKX2A antibody
    • T EBP antibody
    • T/EBP antibody
    • TEBP antibody
    • Thyroid nuclear factor 1 antibody
    • Thyroid nuclear factor antibody
    • Thyroid specific enhancer binding protein antibody
    • Thyroid transcription factor 1 antibody
    • Tin man antibody
    • Tinman antibody
    • TITF 1 antibody
    • TITF1 antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis using ab137061 showing positive staining in Lung squamous carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland papillary carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis using ab137061 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis using ab137061 showing positive staining in Normal lung tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).

    Immunohistochemical analysis using ab137061 showing positive staining in Small cell lung carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).

    Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland tissue.

     

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
    Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab227576? Please let us know so that we can cite the reference in this datasheet.

ab227576 has been referenced in 1 publication.

  • Huang Q  et al. Preclinical Modeling of KIF5B-RET Fusion Lung Adenocarcinoma. Mol Cancer Ther 15:2521-2529 (2016). PubMed: 27496134

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab227576.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com