RecombinantRabMAb

Recombinant Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
  • Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free
    See all TTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8190-138] to TTF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment.

  • Positive control

    • Human fetal lung, Human lung adenocarcinoma, Rat and Mouse lung tissue lysates; Human lung carcinoma and Human papillary thyroid carcinoma, Lung squamous carcinoma, Small cell lung carcinoma, Thyroid gland, Thyroid gland papillary carcinoma and Normal lung tissues

  • General notes

    Ab227576 is the carrier-free version of ab137061. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8190-138

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.

    • Tissue specificity

      Thyroid and lung.

    • Involvement in disease

      Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.

    • Sequence similarities

      Belongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Post-translational
      modifications

      Phosphorylated on serine residues.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis using ab137061 showing positive staining in Lung squamous carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland papillary carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis using ab137061 showing negative staining in Normal liver tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis using ab137061 showing positive staining in Normal lung tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab137061 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137061).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).

      Immunohistochemical analysis using ab137061 showing positive staining in Small cell lung carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

      This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-138, in a different buffer formulation (cat# ab137061).

      Immunohistochemical analysis using ab137061 showing positive staining in Thyroid gland tissue.

       

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)
      Anti-TTF1 antibody [EPR8190-138] - BSA and Azide free (ab227576)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab227576? Please let us know so that we can cite the reference in this datasheet.

    ab227576 has been referenced in 1 publication.

    • Huang Q  et al. Preclinical Modeling of KIF5B-RET Fusion Lung Adenocarcinoma. Mol Cancer Ther 15:2521-2529 (2016). PubMed: 27496134

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