Overview

  • Product name

    Anti-TTF1 antibody [EPR8190-6]
    See all TTF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8190-6] to TTF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control

    • HeLa, Human lung adenocarcinoma, fetal lung, mouse lung and rat lung lysates; Human papillary thyroid carcinoma and lung carcinoma tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8190-6
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
    • Tissue specificity

      Thyroid and lung.
    • Involvement in disease

      Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
    • Sequence similarities

      Belongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications

      Phosphorylated on serine residues.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Images

    • ab133638 showing positive staining in Lung adenocarcinoma tissue.

    • Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution
    • All lanes : Anti-TTF1 antibody [EPR8190-6] (ab133638) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Human lung adenocarcinoma lysate
      Lane 3 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • All lanes : Anti-TTF1 antibody [EPR8190-6] (ab133638) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate


      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution
    • ab133638 showing positive staining in Thyroid gland tissue.

    • ab133638 showing negative staining in Normal liver tissue.

    • ab133638 showing positive staining in Small cell lung carcinoma tissue.

    • ab133638 showing negative staining in Lung squamous carcinoma tissue.

    • ab133638 showing negative staining in Normal lung tissue.

    References

    This product has been referenced in:

    • Kopparam J  et al. RIP4 inhibits STAT3 signaling to sustain lung adenocarcinoma differentiation. Cell Death Differ 24:1761-1771 (2017). Read more (PubMed: 28574510) »
    • Dai YB  et al. Ablation of Liver X receptors a and ß leads to spontaneous peripheral squamous cell lung cancer in mice. Proc Natl Acad Sci U S A 113:7614-9 (2016). Read more (PubMed: 27335465) »
    See all 5 Publications for this product

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