Recombinant
RabMAb

Recombinant Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

Overview

  • Product name

    Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free
    See all TTF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8190-6] to TTF1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control

    • HeLa, Human lung adenocarcinoma, fetal lung, mouse lung and rat lung lysates; Human papillary thyroid carcinoma and lung carcinoma tissues
  • General notes

    Ab227575 is the carrier-free version of ab133638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227575 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8190-6
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
    • Tissue specificity

      Thyroid and lung.
    • Involvement in disease

      Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
    • Sequence similarities

      Belongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications

      Phosphorylated on serine residues.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133638 showing positive staining in Thyroid gland tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133638 showing negative staining in Normal liver tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133638 showing positive staining in Small cell lung carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133638 showing negative staining in Lung squamous carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133638 showing negative staining in Normal lung tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-6, in a different buffer formulation (cat# ab133638).

      ab133638 showing positive staining in Lung adenocarcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-6, in a different buffer formulation (cat# ab133638).

      Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Kopparam J  et al. RIP4 inhibits STAT3 signaling to sustain lung adenocarcinoma differentiation. Cell Death Differ 24:1761-1771 (2017). Read more (PubMed: 28574510) »
    • Dai YB  et al. Ablation of Liver X receptors a and ß leads to spontaneous peripheral squamous cell lung cancer in mice. Proc Natl Acad Sci U S A 113:7614-9 (2016). Read more (PubMed: 27335465) »
    See all 5 Publications for this product

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