RecombinantRabMAb

Recombinant Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
  • Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8190-6] to TTF1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free
    See all TTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8190-6] to TTF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control

    • HeLa, Human lung adenocarcinoma, fetal lung, mouse lung and rat lung lysates; Human papillary thyroid carcinoma and lung carcinoma tissues

  • General notes

    Ab227575 is the carrier-free version of ab133638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227575 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8190-6

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.

    • Tissue specificity

      Thyroid and lung.

    • Involvement in disease

      Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.

    • Sequence similarities

      Belongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Post-translational
      modifications

      Phosphorylated on serine residues.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      ab133638 showing positive staining in Thyroid gland tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      ab133638 showing negative staining in Normal liver tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      ab133638 showing positive staining in Small cell lung carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      ab133638 showing negative staining in Lung squamous carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      ab133638 showing negative staining in Normal lung tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133638).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-6, in a different buffer formulation (cat# ab133638).

      ab133638 showing positive staining in Lung adenocarcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

      This IHC data was generated using the same anti-TTF1 antibody clone, EPR8190-6, in a different buffer formulation (cat# ab133638).

      Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
      Anti-TTF1 antibody [EPR8190-6] - BSA and Azide free (ab227575)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab227575? Please let us know so that we can cite the reference in this datasheet.

    ab227575 has been referenced in 5 publications.

    • Kopparam J  et al. RIP4 inhibits STAT3 signaling to sustain lung adenocarcinoma differentiation. Cell Death Differ 24:1761-1771 (2017). PubMed: 28574510
    • Dai YB  et al. Ablation of Liver X receptors a and ß leads to spontaneous peripheral squamous cell lung cancer in mice. Proc Natl Acad Sci U S A 113:7614-9 (2016). PubMed: 27335465
    • Han X  et al. Transdifferentiation of lung adenocarcinoma in mice with Lkb1 deficiency to squamous cell carcinoma. Nat Commun 5:3261 (2014). IHC-P ; Mouse . PubMed: 24531128
    • Vose LR  et al. Prenatal betamethasone does not affect glutamatergic or GABAergic neurogenesis in preterm newborns. Neuroscience 270:148-57 (2014). PubMed: 24735821
    • Tchaicha JH  et al. Kinase domain activation of FGFR2 yields high-grade lung adenocarcinoma sensitive to a Pan-FGFR inhibitor in a mouse model of NSCLC. Cancer Res 74:4676-84 (2014). PubMed: 25035393

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