Key features and details
- Rabbit polyclonal to TTF1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTF1 antibody - N-terminal
See all TTF1 primary antibodies
DescriptionRabbit polyclonal to TTF1 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TTF1 (N terminal). The exact sequence is proprietary.
Database link: Q15361
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates.
This product was previously labelled as Transcription termination factor 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229565 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 103 kDa.|
FunctionMultifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
Sequence similaritiesContains 2 Myb-like domains.
DomainThe N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus > nucleolus.
- Information by UniProt
- RNA polymerase I termination factor antibody
- Transcription termination factor 1 antibody
- Transcription termination factor I antibody
All lanes : Anti-TTF1 antibody - N-terminal (ab229565) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 103 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229565 has not yet been referenced specifically in any publications.